Iso-Insulin ELISA

(10-1128-01)

Kit format: 1×96 wells

Measurement range: 3 - 100 mU/L (0.13 - 4.35 µg/L)

1.Measures total insulin

2.Cross-reactivity with most insulin analogs

3.Calibrated against 1st International Reference Preparation 66/304 for human insulin.

4.CE/IVD labeled on select markets

5.Controls available: 10-1134-01 (US only) or 10-1164-01

DESCRIPTION

Mercodia Iso-Insulin ELISA provides a method for the quantitative determination of total insulin.

It is a high quality enzyme immunoassay for the quantification of insulin; both endogenous and exogenous. The assay measures common, as well as non-commercially available, insulin analogues and comes with full cross-reactivity data.

Combination of Mercodia Iso-Insulin ELISA with the highly specific Mercodia Insulin ELISA enables calculation of analog concentrations by subtracting endogenous insulin.

TEST CHARACTERISTICS

SPECIES：Human

SAMPLES：Serum, plasma, cell culture medium

SAMPLE VOLUME：25µL

ASSAY RANGE：3 - 100 mU/L (0.13 - 4.35 µg/L)

DETECTION LIMIT：≤ 2 mU/L (0.086 µg/L)

INCUBATION：1h + 15 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

INSULIN：100 %

C-PEPTIDE：<0.1 %

PROINSULIN：54 %

PROINSULIN DES (31-32)：58 %

PROINSULIN SPLIT (32-33):56 %

PROINSULIN DES (64-65)：66 %

PROINSULIN SPLIT (65-66)：78 %

INSULIN ASPART:100%

INSULIN DETEMIR：28%

INSULIN GLARGIN：58%

INSULIN GLULISINE：128%

INSULIN LISPRO：Not detected

IGF-I：<0.002 %

IGF-II：<0.002 %

RAT INSULIN：71%

MOUSE INSULIN：49 %

PORCINE INSULIN：76 %

OVINE INSULIN：131 %

BOVINE INSULIN：58 %