Porcine C-Peptide ELISA

(10-1256-01)

Kit format: 1×96 wells

Measurement range: 20-1200 pmol/L

1.No cross reactivity to human proinsulin or insulin

2.No cross reactivity to C-peptide in human, macaque, rat or mouse

3.High specificity - enables specific measurement of porcine C-peptide in xenotransplantation studies

DESCRIPTION

An assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

The assay may be used for post-transplant assessment of porcine islet xenograft function in human, macaque, rat or mouse. Enables distinguishing endogenously produced C-peptide for pharmacodynamic and/or pharmacokinetic studies during exogenous insulin administration

TEST CHARACTERISTICS

SPECIES：Porcine

SAMPLES：Serum, plasma and cell culture medium

SAMPLE VOLUME：10µL

ASSAY RANGE：20-1200 pmol/L

DETECTION LIMIT：≤10 pmol/L

INCUBATION TIME：2 h + 1 h + 15 min

DETECTION METHOD：Absorbance

SPECIFICITY

The following cross-reactions have been tested:

PORCINE INSULIN：≤ 0.05 %

PORCINE PROINSULIN：<0.02 %

HUMAN C-PEPTIDE：<0.003 %

HUMAN INSULIN：<0.002 %

HUMAN PROINSULIN：≤ 0.01 %

MACAQUE C-PEPTIDE：<0.006 %

MOUSE INSULIN：<0.13 %

MOUSE PROINSULIN：<0.002 %

RAT C-PEPTIDE：0.08 %

RAT INSULIN：<0.002 %

RAT PROINSULIN：<0.002 %