ELISA AP Substrate

Lumi-Phos 530 is a ready-to-use chemiluminescent reagent formulation containing the alkaline phosphatase (AP) substrate, Lumigen PPD. Lumi-Phos 530 is useful for chemiluminescent detection of AP conjugates in immunoassays and DNA probe assays. It provides ultrasensitive detection of analytes captured on magnetic particles and in microtiter plate wells. Lumi-Phos 530 is 10,000 times more sensitive than colorimetric substrates in solution. 

• Excellent sensitivity - low picogram to femtogram
• Sustained luminescence - with low concentrations of AP, the light intensity remains constant for several hours
• Linear calibration curves - the luminescence intensity at any time point is a direct measure of the concentration of the enzyme
• High stability and long shelf life

Chemiluminescent Enhancement

A unique co-surfactant system promotes and enhances energy-transfer to the fluorescent head groups causing the generation of luminescence with a broad emission maximum at 530 nm.